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standard antisera |
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General:
These antisera have a very high avidity and titre, which makes
them ideal even for liquid-phase immunoprecipitation techniques,
immunoturbidimetry and immunonephelometry.
Specificity:
This is controlled by immunoelectrophoresis against human serum
twice concentrated and enriched with antigens that are normally
absent or present at low concentrations in human serum.
Title: the
variation of the titre from one lot to another is below 10%.
Form: liquid.
Stability: more than
one year at 2-8 ºC.
Applications:
• Qualitative:
- Immunoprecipitation in gel: immunoelectrophoresis,
immunofixation, etc.
- First antibody in indirect techniques: immunofluorescence,
immunoenzymatic, etc.
• Quantitative:
- Immunoprecipitation in gel: radial immunodiffusion,
electroimmunoprecipitation (Laurell), etc.
- Liquid-phase immunoprecipitation: turbidimetry,
nephelometry.
Unless specified otherwise, all the antisera are of goat anti-human
proteins. |
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| Albumin |
2 ml |
 |
A.ALB.STR |
| Alfa-1 Antitrypsin |
2 ml |
|
A.AAT.STR |
| Alfa-1 Acid Glicoprotein (orosomucoid) |
2 ml |
|
A.AGP.STR |
| Alfa-2 Macroglobulin |
2 ml |
|
A.A2M.STR |
| Antithrombin III |
2 ml |
|
A.AT3.STR |
| Beta-2 Microglobulin |
2 ml |
|
A.B2M.STR |
| Ceruloplasmin |
2 ml |
|
A.CER.STR |
| Complement C1 (esterase) Inhibitor |
2 ml |
|
A.CEI.STR |
| Complement C1q |
2 ml |
|
A.C1Q.STR |
| Complement C3 (Beta1C/Beta1A/C3c) |
2 ml |
|
A.CC3.STR |
| Complement C4 (Beta1E) |
2 ml |
|
A.CC4.STR |
| Ferritin |
2 ml |
|
A.FER.STR |
| Fibrinogen |
2 ml |
|
A.FIB.STR |
| Fibronectin |
2 ml |
|
A.FIN.STR |
| Haptoglobin |
2 ml |
|
A.HPT.STR |
| Immunoglobulins IgA (Alpha Chains) |
2 ml |
|
A.IGA.STR |
| Immunoglobulins IgG (Fc Fragment) |
2 ml |
|
A.IGG.STR |
| Immunoglobulins IgM (Mu Chains) |
2 ml |
|
A.IGM.STR |
| Lipoprotein (a) |
2 ml |
|
A.LPA.STR |
| Prealbumin (TBPA, Prealb. Binding Tiroxin) |
2 ml |
|
A.PAL.STR |
| Protein C-Reactive |
2 ml |
|
A.CRP.STR |
| Transferrin |
2 ml |
|
A.TRF.STR |
|
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|
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|
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|
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purified IgG fraction antisera |
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|
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General:
The IgG fraction is prepared by chromatographic separation of
the IgGs of standard antisera.
This procedure removes most of the non-specific proteins from
the antiserum and gives a reagent that is made almost exclusively
of antibodies.
This determines a minor tendency to aggregation of the antibodies
and, consequently, greater stability and, above all, a low unspecific
reagent background, which is particularly important in reactions
in gel precipitation.
They have a very high avidity and titre, which makes them ideal
even for liquid-phase immunoprecipitation techniques, immunoturbidimetry
and immunonephelometry.
Especificity:
This is controlled by immunoelectrophoresis against human serum
twice concentrated and enriched with antigens that are normally
absent or present at low concentrations in human serum.
Title:
the variation of the titre from one lot to another is below
10%.
Form: liquid.
Stability: more than
one year at 2-8 ºC.
Applications:
• Qualitative:
- Immunoprecipitation in gel: immunoelectrophoresis,
immunofixation, etc.
- First antibody in indirect techniques: immunofluorescence,
immunoenzymatic, etc.
• Quantitative:
- Immunoprecipitation in gel: radial immunodiffusion,
electroimmunoprecipitation (Laurell), etc.
- Liquid-phase immunoprecipitation: turbidimetry,
nephelometry.
Unless specified otherwise, all the antisera are of goat anti-human
proteins.
|
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fluorescent conjugated antisera |
| |
|
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General:
Fluorescein Isothiocyanate (FITC), isomer I, is conjugated with
the purified IgG fraction of the antiserum.
The free fluorochrome is removed by gel filtration and the optimum
flurochrome/protein (F/P) ratio is ensured by ionic exchange chromatography.
Especificity:
This is controlled by immunoelectrophoresis against human serum
twice concentrated and enriched with antigens that are normally
absent or present at low concentrations in human serum.
Title:
the variation of the titre from one lot to another is below
10%.
Form: liquid.
Stability: more than
one year at 2-8 ºC.
Applications:
Immunohistochemistry, flow cytometry, biopsies, quantitative fluorescence.
Unless specified otherwise, all the antisera are of goat anti-human
proteins. |
| |
|
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|
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|
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Complement C4 (Beta1E)
|
2 ml |
|
A.CC4.FITC |
| Fibrinogen |
2 ml |
|
A.FIB.FITC |
| Immunoglobulins IgA (Alpha Chains) |
2 ml |
|
A.IGA.FITC |
| Immunoglobulins IgM (Mu Chains) |
2 ml |
|
A.IGM.FITC |
|
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immunofixation and immunoelectrophoresis |
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|
| |
General:
This chapter groups together certain antisera that are particularly
used for immunofixation or immunoelectrophoresis.
They are all purified IgG fraction antisera.
For other antisera that can be used for immunofixation, please see
the chapter titled “Purified IgG Fraction Antisera”.
Unless specified otherwise, all the antisera are of goat anti-human
proteins. |
| |
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|
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|
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| Beta-2 Microglobulin |
2 ml |
|
A.B2M.IGG |
| Fibrinogen |
2 ml |
|
A.FIB.IGG |
| rabbit anti Free Kappa Light Chains |
2 ml |
|
A.FRK.IGG |
| rabbit anti Free Lambda Light Chains |
2 ml |
|
A.FRL.IGG |
rabbit anti Free Light Chains
Kappa and Lambda (mixed)
|
2 ml |
|
A.FKL.IGG |
| Immunoglobulins IgA (Alpha Chains) |
2 ml |
|
A.IGA.IGG |
| Immunoglobulins IgG (Fc Fragment) |
2 ml |
|
A.IGG.IGG |
| Immunoglobulins IgM (Mu Chains) |
2 ml |
|
A.IGM.IGG |
Immunoglobulins Kappa
(Kappa Light Chains Bound and Free (B&F))
|
2 ml |
|
A.KAP.IGG |
Immunoglobulins Lambda
(Lambda Light Chains Bound and Free (B&F))
|
2 ml |
|
A.LAM.IGG |
| Protein C-Reactive |
2 ml |
|
A.CRP.IGG |
| rabbit anti Retinol Binding Protein |
2 ml |
|
A.RBP.IGG |
| Transferrin |
2 ml |
|
A.TRF.IGG |
|
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